What question did this study set out to answer?

Determine the quantitative association constants between the A protein of human lactose synthetase and monosaccharide ligands using affinity chromatography.

December 1, 1973Biochemical JournalOpen Access

Use of affinity chromatography for the quantitative study of acceptor–ligand interactions: the lactose synthetase system (Short Communication)

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Authors

PAP AndrewsUniversity of Liverpool BKBarry J. KitchenMonash Health DWDonald J. WinzorThe University of Queensland

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Implication

Biochemical analysis reveals distinct binding affinities for human lactose synthetase A protein via affinity chromatography, suggesting a viable approach for measuring enzyme-ligand interactions.

Key Points

Determine the quantitative association constants between the A protein of human lactose synthetase and monosaccharide ligands using affinity chromatography.
Eluted human lactose synthetase A protein through a Sepharose-alpha-lactalbumin column in the presence of competing monosaccharide ligands.
Calculated association constants based on the elution volume changes caused by N-acetyl-D-glucosamine and D-glucose.
Deduced an association constant of 200 M⁻¹ for the interaction between the lactose synthetase A protein and N-acetyl-D-glucosamine.
Deduced an association constant of 0.57 M⁻¹ for the interaction between the lactose synthetase A protein and D-glucose.

Cite This Study

Andrews et al. (1973) studied this question.

synapsesocial.com/papers/6a7a86debdf697b3de02ab5a https://doi.org/10.1042/bj1350897

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Implication

Biochemical analysis reveals distinct binding affinities for human lactose synthetase A protein via affinity chromatography, suggesting a viable approach for measuring enzyme-ligand interactions.

Use of affinity chromatography for the quantitative study of acceptor–ligand interactions: the lactose synthetase system (Short Communication)

Discussion

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Implication

Key Points

Cite This Study

Use of affinity chromatography for the quantitative study of acceptor–ligand interactions: the lactose synthetase system (Short Communication)

Discussion

Member takes

Implication

Key Points

Cite This Study

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