When Dictyostelium discoideum actin is synthesized in a rabbit reticulocyte lysate system, it is made as a 43,000-dalton polypeptide with the NHz-terminal sequence Ac-Met-Asp-Gly . . .even though in vivo the NHz-terminal sequence is Ac-Asp-Gly . . .
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Redman et al. (1981) studied this question.
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