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June 17, 1977Science1,256 citations

Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

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AUAxel UllrichJSJohn ShineJCJohn M. Chirgwin

Key Points

  • The aim is to construct plasmids that contain rat insulin gene coding sequences for further analysis.
  • Constructed recombinant bacterial plasmids containing complementary DNA from rat islets of Langerhans mRNA.
  • Cloned sequences represent the complete coding region of rat proinsulin I, part of preproinsulin I prepeptide, and the 3' terminal region of mRNA.
  • A fourth plasmid includes sequences from the A chain region of rat preproinsulin II.
  • Successfully created three plasmids with the complete coding region of rat proinsulin I.
  • Developed one plasmid including the A chain region from rat preproinsulin II.
  • Plasmids could aid in understanding insulin gene expression.

Abstract

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.

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Cite This Study

Ullrich et al. (1977) studied this question.

synapsesocial.com/papers/6a09794c59b902245b45d163https://doi.org/10.1126/science.325648
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