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February 1, 1983Proceedings of the National Academy of SciencesOpen Access

In vivo expression of rat insulin after intravenous administration of the liposome-entrapped gene for rat insulin I.

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Authors

CNC. NicolauDemosAPA. Le PapeSt. Lawrence UniversityPSPhilippe SorianoSouthern Illinois University Carbondale

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Implication

Randomized trial demonstrates enhanced insulin production in rats using liposome-encapsulated gene delivery, suggesting new therapeutic avenues.

Key Points

  • The aim is to evaluate the effects of liposome-entrapped rat insulin gene delivery on insulin expression and glycemia in rats.
  • Intravenous injection of liposome-encapsulated recombinant plasmid encoding rat preproinsulin I in rats.
  • Comparison with controls including empty liposomes and various plasmids.
  • Measurement of blood glucose and insulin levels at several time points post-injection.
  • Treated rats had a blood glucose level of 72 +/- 5 mg/100 ml compared to 107 +/- 2 mg/100 ml for controls (p<0.001).
  • Blood insulin levels in treated rats were 61 +/- 8 microunits/ml versus 43 +/- 5 microunits/ml for controls (p<0.005).
  • Spleen and liver insulin levels in treated rats were significantly higher at 242 +/- 22 and 204 +/- 20 microunits/g versus 112 +/- 20 and 87 +/- 15 microunits/g in controls (p<0.05).

Cite This Study

Nicolau et al. (1983) studied this question.

synapsesocial.com/papers/6a156f0b814bf8ec9a4e95a4https://doi.org/10.1073/pnas.80.4.1068
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