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April 1, 1996The Journal of Immunology

Differential regulation of IL-6 gene transcription and expression by IL-4 and IL-10 in human monocytic cell lines

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Authors

STSehchiro TakeshitaUCLA HealthJGJulia R. GageMoorpark CollegeTKT KishimotoTokai University

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Implication

In vitro study demonstrates distinct transcriptional and post-transcriptional suppression of IL-6 by IL-4 and IL-10 in human monocytic cells, highlighting divergent anti-inflammatory mechanisms.

Key Points

  • To determine the molecular mechanisms by which IL-4 and IL-10 inhibit IL-6 gene expression at the transcriptional or post-transcriptional level in human monocytic cells.
  • Assessed IL-6 protein levels, mRNA expression, and mRNA stability in PMA- and LPS-stimulated THP-1 and U937 human monocytic cell lines treated with or without IL-4 or IL-10.
  • Measured IL-6 promoter transcriptional activity using chloramphenicol acetyltransferase reporter assays and evaluated nuclear NF-kappaB and NF-IL-6 binding using electrophoretic mobility shift assays.
  • Both IL-4 and IL-10 inhibited IL-6 cytokine production and IL-6 mRNA expression in stimulated THP-1 and U937 cell lines.
  • IL-4 suppressed stimulated IL-6 gene promoter activity and nuclear NF-kappaB binding without altering NF-IL-6, whereas IL-10 showed no inhibitory effect on promoter activity or transcription factor binding.
  • IL-10 enhanced the degradation rate of IL-6 mRNA in stability assays, indicating post-transcriptional suppression.

Cite This Study

Takeshita et al. (1996) studied this question.

synapsesocial.com/papers/6a7aa66da861e9f59932aee6https://doi.org/10.4049/jimmunol.156.7.2591
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