Why the study?
Cardiotoxicity is a major concern following cancer treatment, making preclinical prediction of cardiotoxicity important, whereas current models are limited to cell culture or rodent models.
Daphnia magna serves as a simple, transparent invertebrate model for rapid preclinical screening of cancer chemotherapeutic cardiotoxicity.
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Daphnia magna model enables rapid cardiotoxicity screening; leaves open validation and translation to mammalian or clinical prediction.
One of the major concerns following cancer treatment is cardiotoxicity. Therefore, it is important to predict potential cardiotoxicity of cancer chemotherapeutics at the preclinical phase. Current models of cardiotoxicity testing involve either cell culture models or rodent models. We developed a simple invertebrate animal model for rapid screening of cardiotoxicity of cancer chemotherapeutics. Daphnia magna (water flea, a crustacean) has a transparent body and a large myogenic heart that can be easily monitored under a microscope. Using this model, we have previously described comparative cardiotoxicity of several kinase inhibitors that were approved for the treatment of multiple cancers. In this article, we describe the step-wise protocols for evaluating the heart rate and survival of D. magna with relevant information on troubleshooting.
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Mohammed et al. (2024) studied this question.
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