What question did this study set out to answer?

To characterize the carbon-13 chemical shifts of small peptides as a function of pH and evaluate the effects of amino acid sequence and position.

June 1, 1972Journal of the American Chemical Society

Nuclear magnetic resonance spectroscopy. Carbon-13 chemical shifts of small peptides as a function of pH

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Authors

MCManfred ChristlUniversity of Bergamo JRJohn D. RobertsYale University

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Implication

Spectroscopic analysis reveals position- and pH-dependent carbon-13 chemical shifts across 27 peptides, suggesting a reliable method for determining short peptide sequences.

Key Points

To characterize the carbon-13 chemical shifts of small peptides as a function of pH and evaluate the effects of amino acid sequence and position.
Obtained carbon-13 spectra for 27 peptides using high-resolution nuclear magnetic resonance (NMR) spectroscopy with proton decoupling.
Analyzed chemical shift deviations across protonated, zwitterionic, and deprotonated states as a function of peptide position and neighboring residues.
Chemical shift deviations from free zwitterionic amino acids depended strongly on chain position (N-terminal, C-terminal, or internal) but were largely independent of neighboring residues, unless adjacent to proline.
Protonation and deprotonation primarily affected the chemical shifts of the directly reacting terminal units, with exceptions reflecting conformational changes between ionic states.

Cite This Study

Christl et al. (1972) studied this question.

synapsesocial.com/papers/6a7ac0584353fbf06002272f https://doi.org/10.1021/ja00768a026

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Implication

Spectroscopic analysis reveals position- and pH-dependent carbon-13 chemical shifts across 27 peptides, suggesting a reliable method for determining short peptide sequences.

Nuclear magnetic resonance spectroscopy. Carbon-13 chemical shifts of small peptides as a function of pH

Discussion

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Implication

Key Points

Cite This Study

Nuclear magnetic resonance spectroscopy. Carbon-13 chemical shifts of small peptides as a function of pH

Discussion

Member takes

Implication

Key Points

Cite This Study

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