What question did this study set out to answer?

To investigate the functional interplay between intracellular calcium signaling and tyrosine kinase activation in cultured human endothelial cells.

April 1, 1995Circulation Research

Calcium Signaling in Endothelial Cells Involves Activation of Tyrosine Kinases and Leads to Activation of Mitogen-Activated Protein Kinases

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To investigate the functional interplay between intracellular calcium signaling and tyrosine kinase activation in cultured human endothelial cells.
Stimulated cultured human umbilical vein endothelial cells with bradykinin (100 nmol/L), histamine (1 μmol/L), or thapsigargin (30 nmol/L) in the presence or absence of extracellular Ca2+.
Identified phosphorylated target proteins by immunoprecipitation and tested kinase dependence using tyrosine kinase inhibitors genistein (100 μmol/L) and piceatannol (10 μmol/L).
Monitored intracellular Ca2+ transients using fura-2 fluorometry under receptor-dependent and receptor-independent activation.
Agonist stimulation induced delayed, prolonged tyrosine phosphorylation of 42-kD and 44-kD isoforms of MAP kinase.
MAP kinase phosphorylation was abolished by extracellular Ca2+ depletion or treatment with genistein and piceatannol.
Tyrosine kinase inhibition selectively attenuated the sustained plateau phase of Ca2+ signaling, demonstrating that extracellular Ca2+ influx is modulated by tyrosine phosphorylation.

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Implication

In vitro study demonstrates mutual dependence between calcium influx and tyrosine kinase-dependent MAP kinase activation in cultured endothelial cells, indicating coupled signaling pathways.

Cite This Study

Fleming et al. (1995) studied this question.

synapsesocial.com/papers/6a7ac17d14b028facf0e88a0 https://doi.org/10.1161/01.res.76.4.522

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Implication

In vitro study demonstrates mutual dependence between calcium influx and tyrosine kinase-dependent MAP kinase activation in cultured endothelial cells, indicating coupled signaling pathways.

Calcium Signaling in Endothelial Cells Involves Activation of Tyrosine Kinases and Leads to Activation of Mitogen-Activated Protein Kinases

Key Points

Discussion

Implication

Cite This Study

Calcium Signaling in Endothelial Cells Involves Activation of Tyrosine Kinases and Leads to Activation of Mitogen-Activated Protein Kinases

Key Points

Discussion

Implication

Cite This Study

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