April 14, 2009PLoS BiologyOpen Access

Coordinated Movement of Cytoplasmic and Transmembrane Domains of RyR1 upon Gating

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Population

Frozen-hydrated RyR1-FKBP12 particles (n=15,625 in closed conformation and n=18,527 in open conformation)

Design

Preclinical

Authors

Discussion

Structural details of RyR1 gating in animal models may guide future research; leaves open translation to human cardiac RyR2 and therapies.

Population

Frozen-hydrated RyR1-FKBP12 particles (n=15,625 in closed conformation and n=18,527 in open conformation)

Intervention

EGTA or neuroactive PCB 95 to stabilize the full closed or open states

Outcome

Three-dimensional structures at 10.2 A resolution and gating mechanismsurrogate

Reveals the structural basis of RyR1 channel gating, demonstrating coordinated movement of cytoplasmic and transmembrane domains.

Samsó et al. (2009) studied this question.

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Structural details of RyR1 gating in animal models may guide future research; leaves open translation to human cardiac RyR2 and therapies.