What question did this study set out to answer?

To develop a cost-effective barcoding strategy for full-length 16S rRNA sequencing to improve species-level identification in environmental samples.

August 11, 2026BMC GenomicsOpen Access

A hybrid and cost-efficient barcoding strategy for full-length 16S rRNA gene nanopore sequencing of environmental samples

View Full Paper

Ask AI

Bookmark

Authors

Randomized trial evaluates a new barcoding strategy for enhancing taxonomic resolution in environmental samples, suggesting cost-effective solutions.

To develop a cost-effective barcoding strategy for full-length 16S rRNA sequencing to improve species-level identification in environmental samples.
Implemented a hybrid barcoding workflow by combining PacBio PCR-based barcoding with ONT library preparation.
Utilized environmental samples, including natural soil and cultured microbial communities.
Developed a new bioinformatic workflow tailored for ONT sequencing data.
Achieved per-sample barcoding cost reduction while maintaining high sequencing quality (5 Gb of quality-filtered data).
Taxonomic assignment achieved for 49.38% of annotated taxa, significantly higher than Illumina NovaSeq's 4.59%.
Recovered 2.3 times more genera and 1.3 times more families compared to short-read sequencing.

Romano et al. (2026) studied this question.

View Full Paper

Ask AI

Bookmark

Randomized trial evaluates a new barcoding strategy for enhancing taxonomic resolution in environmental samples, suggesting cost-effective solutions.