Key result
Preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated pulmonary hypertension development without altering col1a1 and myh7 gene expression in rats with PAH.
Why the study?
The study was conducted to gain insight into the impact of preventive exercise on gene expression of myosins and extracellular matrix remodeling during pulmonary arterial hypertension.
Does preventive exercise improve functional capacity and cardiac remodeling in a rat model of pulmonary arterial hypertension?
Does preventive exercise improve functional capacity and cardiac remodeling in a rat model of pulmonary arterial hypertension?
Preventive exercise improves functional capacity and attenuates right ventricular hypertrophy in a rat model of pulmonary arterial hypertension, independent of changes in col1a1 and myh7 gene expression.
Hypothesis-generating for preventive exercise in experimental PAH; clinical translation and mechanisms require prospective study.
To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton's index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton's index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH.
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Mariano et al. (2021) studied Pulmonary arterial hypertension (n=32). Preventive exercise (treadmill training) vs. Sedentary control was evaluated on Gene expression of myosins and extracellular matrix remodeling, functional capacity, and cardiac hypertrophy. Preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated pulmonary hypertension development without altering col1a1 and myh7 gene expression in rats with PAH.
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