Components of the cell envelope of Vibrio parahaemolyticus were investigated. Vibrio parahaemolyticus is an estuarine microorganism associated with diseases of marine and estuarine animals and seafood-borne enteritis in man. Purified lipopolysaccharide (LPS), isolated using a 45% phenol extraction procedure, was found to contain lipid A fraction to 27% of the LPS by weight. In the lipid A fraction, glucosamine was the only amino sugar to be present and a high molar ratio of phosphate to amino sugar (2.5:1) was noted. Two hydroxy fatty acids, hydroxydodecanoic and hydroxymyristic, were identified among the fatty acids by gas–liquid chromatography. A role of the lipopolysaccharides in the salt requirement of marine bacteria is suggested.
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Deneke et al. (1973) studied this question.