October 1, 1997Cardiovascular ResearchOpen Access

Role of endothelin, nitric oxide and L-arginine release in ischaemia/reperfusion injury of rat heart

View Full Paper

Ask AI

Bookmark

Why the study?

Does modulation of NO and ET-1 pathways improve cardiac function and reduce injury in a rat heart model of ischaemia/reperfusion?

Population

Rat hearts perfused at 9 ml/min per g, subjected to 15 min total global ischaemia and reperfused for 30 min

Comparison

L-arginine, the NO donor S-nitroso-N-acetyl-DL-pe… vs Vehicle administered from 5 min pre-ischaemia to…

Design

Preclinical

Follow-up

30 minutes of reperfusion

Authors

FBFriedrich BrünnerUniversität Innsbruck

Discussion

Member takes

Implication

NO/ET-1 modulation improves rat reperfusion function; leaves open translation to human cardioprotection.

Structured PICO

Does modulation of NO and ET-1 pathways improve cardiac function and reduce injury in a rat heart model of ischaemia/reperfusion?

Population

Rat hearts (n=25 total, 5 groups of n=5 each) perfused at 9 ml/min per g, subjected to 15 min total global ischaemia and reperfused for 30 min

Intervention

L-arginine (1 mmol/l), the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP; 200 mumol/l), the inhibitor of NO formation NG-nitro-L-arginine (L-NNA; 200 mumol/l), or the ET receptor antagonist PD 142893 (200 nmol/l) administered from 5 min pre-ischaemia to end of reperfusion

Comparator

Vehicle administered from 5 min pre-ischaemia to end of reperfusion

Outcome

Cardiac function (systolic, diastolic, and coronary reperfusion function) and release of L-arginine, cyclic GMP, and lactate dehydrogenase (LDH) into coronary effluentsurrogate

In a rat model of ischaemia/reperfusion, ET-1 causes cell necrosis and L-arginine outflow without compromising NO synthesis, while NO donors and ET receptor antagonists improve cardiac function.

Cite This Study

Friedrich Brünner (1997) studied this question.

synapsesocial.com/papers/6a7b586cb008f0cadf329e32 https://doi.org/10.1016/s0008-6363(97)00138-7

View Full Paper

Ask AI

Bookmark

Role of endothelin, nitric oxide and L-arginine release in ischaemia/reperfusion injury of rat heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Role of endothelin, nitric oxide and L-arginine release in ischaemia/reperfusion injury of rat heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider