Evidence of Hyperacetylation of Mitochondrial Regulatory Proteins in Left Ventricular Myocardium of Dogs with Chronic Heart Failure

Figures

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  Figure 1. Bar graphs (mean ± SEM) of levels of NAD (left), NADH (middle), and NAD+/NADH ratio (right) in isolated mitochondrial fractions of left ventricular (LV) myocardium of seven healthy normal (NL) dogs and seven dogs with heart failure (HF).

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  Figure 2. (Left): Western blots of sirtuin-3 (Sirt3 and Porin) in mitochondrial fractions of seven healthy normal dogs (NL) and seven dogs with heart failure (HF). (Right): Bar graphs (mean ± SEM) depicting protein levels of Sirt3 (left), porin (middle), and the ratio of Sirt3/porin (right) in mitochondrial fractions of LV myocardium of seven healthy NL dogs and seven dogs with HF.

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  Figure 3. (Left) Western blot of CD38 in mitochondrial fractions of seven healthy normal dogs (NL) and seven dogs with LV heart failure (HF). (Right) Bar graphs (mean ± SEM) depicting protein levels of CD38 and the ratio of CD38/porin in mitochondrial fractions of LV myocardium of seven healthy NL dogs and seven dogs with HF.

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  Figure 4. (Left) Western blot of cyclophilin-D (CyPD) in mitochondrial fractions of seven normal dogs (NL) and seven dogs with heart failure (HF). (Right) Bar graphs (mean ± SEM) depicting protein levels of CyPD and the ratio of CyPD/porin in mitochondrial fractions of LV myocardium of seven healthy NL dogs and seven dogs with HF.

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  Figure 5. Bar graphs (mean ± SEM) depicting Complex-1 activity assayed in isolated mitochondrial fractions of LV myocardium of six healthy NL dogs and seven dogs with HF.