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August 1, 1998Journal of Biological ChemistryOpen Access

Role of N-Glycosylation in Human Angiotensinogen

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Population

COS-7 and/or CHO-K1 cells expressing human angiotensinogen

Comparison

Systematic site-directed mutagenesis of four… vs Wild-type human angiotensinogen

Design

Preclinical

Authors

AGAnne‐Paule Gimenez‐RoqueploDélégation Paris 5JCJérôme CélérierCentre National de la Recherche ScientifiqueGLG. LucarelliSapienza University of Rome

Discussion

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Implication

The N-4 mutant offers a homogeneous, efficient tool for angiotensinogen crystallography; leaves open effects on native function or relevance.

Structured PICO

P
Population
COS-7 and/or CHO-K1 cells expressing human angiotensinogen
I
Intervention
Systematic site-directed mutagenesis (Asn replaced with Gln) of four putative asparagine-linked glycosylation sites (11 mutants produced, including a quadruple N-4 mutant)
C
Comparator
Wild-type human angiotensinogen
O
Outcome
Catalytic efficiency (kcat/Km), protein heterogeneity, and thermal stabilitysurrogate

Deglycosylation of human angiotensinogen via site-directed mutagenesis increases its catalytic efficiency and reduces heterogeneity without affecting thermal stability, providing a useful recombinant protein for x-ray crystallography.

Cite This Study

Gimenez‐Roqueplo et al. (1998) studied this question.

synapsesocial.com/papers/6a7ba243a540d02696c28d13https://doi.org/10.1074/jbc.273.33.21232
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cloning and sequence analysis of cDNA for rat angiotensinogen.1983 · 239 citations
  2. 2Purification and characterization of ovine angiotensinogen1986 · 7 citations
  3. 3Molecular Genetics of Human Hypertension: Role of Angiotensinogen*1997 · 135 citations
  4. 4Measurement of Renin Activity, Concentration and Substrate in Rat Plasma by Radioimmunoassay of Angiotensin I1972 · 455 citations
  5. 5Rapid and efficient site-specific mutagenesis without phenotypic selection.1985 · 7,328 citations