What question did this study set out to answer?

To determine whether glutamate receptor activation modulates presynaptic vesicle recycling and specific presynaptic protein complexes during synapse formation.

September 1, 2001Journal of NeuroscienceOpen Access

Chronic Blockade of Glutamate Receptors Enhances Presynaptic Release and Downregulates the Interaction between Synaptophysin-Synaptobrevin–Vesicle-Associated Membrane Protein 2

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Authors

ABAlberto BacciCentre National de la Recherche Scientifique SCSilvia CocoUniversity of Milano-Bicocca EPElena PravettoniUniversity of Milan

Discussion

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Implication

In vitro study reveals glutamate receptor blockade increases vesicle release in hippocampal neurons, indicating receptor signaling tunes synaptogenesis via synaptophysin-VAMP2 complexes.

Key Points

To determine whether glutamate receptor activation modulates presynaptic vesicle recycling and specific presynaptic protein complexes during synapse formation.
Treated primary hippocampal neuron cultures chronically with NMDA and non-NMDA glutamate receptor antagonists or tetrodotoxin (TTX) during synaptogenesis.
Assessed presynaptic functional changes by recording miniature excitatory postsynaptic currents (mEPSCs) and measuring lumenal synaptotagmin antibody uptake.
Quantified synaptophysin-synaptobrevin-VAMP2 interactions via co-immunoprecipitation and challenged neurons intracellularly with a binding-inhibitory peptide.
Chronic glutamate receptor blockade increased mEPSC frequency and glutamatergic synaptotagmin antibody uptake without altering mEPSC amplitude or synapse density.
Receptor blockade decreased the formation of synaptophysin-synaptobrevin-VAMP2 complexes, whereas competitive inhibition of this complex increased mEPSC frequency selectively in control neurons.
Chronic TTX treatment did not increase vesicle recycling, demonstrating that the presynaptic enhancement was independent of general electrical silence.

Cite This Study

Bacci et al. (2001) studied this question.

synapsesocial.com/papers/6a7bc9120bfa86fad9c63f16 https://doi.org/10.1523/jneurosci.21-17-06588.2001

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Implication

In vitro study reveals glutamate receptor blockade increases vesicle release in hippocampal neurons, indicating receptor signaling tunes synaptogenesis via synaptophysin-VAMP2 complexes.

Chronic Blockade of Glutamate Receptors Enhances Presynaptic Release and Downregulates the Interaction between Synaptophysin-Synaptobrevin–Vesicle-Associated Membrane Protein 2

Discussion

Member takes

Implication

Key Points

Cite This Study

Chronic Blockade of Glutamate Receptors Enhances Presynaptic Release and Downregulates the Interaction between Synaptophysin-Synaptobrevin–Vesicle-Associated Membrane Protein 2

Discussion

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Implication

Key Points

Cite This Study

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