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The disinfectant present in most disinfectant-virus mixtures is inherently toxic to cell cultures and thus precludes the observation of viral inactivation at low dilutions of virus. Therefore, a method employing Sephadex was devised which would remove the cytotoxic activity of virus-disinfectant mixtures. These studies show that Sephadex is an acceptable detoxicant for numerous low molecular weight compounds and does not affect the biological activity of Herpes simplex virus. Data are presented on the effect of low molecular weight chemical compounds, i.e., phenol, ethyl alcohol, quaternary ammonium compounds, and halogens, on H.Ep. 2 cell culture and the macroplaque strain of H. simplex virus.
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Blackwell et al. (1970) studied this question.
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