What question did this study set out to answer?

Evaluate methods for directing stable, physiologically regulated gene expression to rat livers using replication-incompetent retroviral vectors governed by the liver-specific PEPCK promoter.

October 1, 1990Journal of Biological ChemistryOpen Access

Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase.

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Authors

MHMaria HatzoglouUniversity School WLWouter H. LamersAmsterdam UMC Location University of Amsterdam FBFátima BoschUniversitat Autònoma de Barcelona

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Implication

Experimental study demonstrates sustained, hormone-regulated hepatic transgene expression in fetal and adult rats, highlighting the utility of retroviruses for liver-targeted gene therapy.

Key Points

Evaluate methods for directing stable, physiologically regulated gene expression to rat livers using replication-incompetent retroviral vectors governed by the liver-specific PEPCK promoter.
Constructed replication-incompetent retroviral vectors containing the phosphoenolpyruvate carboxykinase (PEPCK) promoter linked to neo or bovine growth hormone (bGH) genes.
Infected rat livers either via in utero intraperitoneal injection in fetal rats or via direct portal vein injection following partial hepatectomy in adult rats.
Retroviral proviruses stably integrated into hepatic DNA, maintaining PEPCK promoter-directed expression for up to 8 months post-infection.
Chimeric PEPCK-bGH gene expression in the liver exhibited physiological regulation by diet and hormones comparable to endogenous PEPCK gene expression.

Cite This Study

Hatzoglou et al. (1990) studied this question.

synapsesocial.com/papers/6a7beff5cbabed012ec62e14 https://doi.org/10.1016/s0021-9258(17)44901-5

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Implication

Experimental study demonstrates sustained, hormone-regulated hepatic transgene expression in fetal and adult rats, highlighting the utility of retroviruses for liver-targeted gene therapy.

Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase.

Discussion

Member takes

Implication

Key Points

Cite This Study

Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase.

Discussion

Member takes

Implication

Key Points

Cite This Study

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