What question did this study set out to answer?

To determine the functional importance of E2F transcription factor binding at endogenous metabolic gene promoters, specifically Phosphoglycerate kinase (Pgk), in Drosophila development.

April 3, 2023Proceedings of the National Academy of SciencesOpen Access

E2F regulation of the Phosphoglycerate kinase gene is functionally important in Drosophila development

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Authors

MZMaría Paula ZappiaUniversity of Illinois Chicago YKYong-Jae KwonUniversity of Illinois Chicago AWAnton WestacottUniversity of Illinois Chicago

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Implication

Experimental genetic study demonstrates that disrupting E2F binding to the Phosphoglycerate kinase promoter impairs metabolism in Drosophila, indicating critical metabolic control by cell cycle...

Key Points

To determine the functional importance of E2F transcription factor binding at endogenous metabolic gene promoters, specifically Phosphoglycerate kinase (Pgk), in Drosophila development.
Generated point mutations in the promoter E2F binding sites of five endogenous metabolic genes in Drosophila melanogaster using CRISPR/Cas9.
Evaluated the Pgk ΔE2F mutant phenotype by measuring target gene expression, glycolytic flux, tricarboxylic acid cycle intermediates, ATP content, chromatin accessibility, and tissue morphology.
Mutation of the E2F site upstream of Pgk markedly reduced gene expression, resulting in decreased glycolytic flux, reduced TCA cycle intermediates, lower ATP content, and abnormal mitochondrial morphology.
Pgk ΔE2F mutants exhibited significantly reduced chromatin accessibility across multiple genomic regions, accompanied by downregulation of hundreds of metabolic and developmental genes.
Loss of E2F regulation at the Pgk locus led to shortened organismal lifespan and structural defects in high-energy organs, including muscles and ovaries.

Cite This Study

Zappia et al. (2023) studied this question.

synapsesocial.com/papers/6a7bfe6b4e26224dc1ed1b3d https://doi.org/10.1073/pnas.2220770120

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Implication

Experimental genetic study demonstrates that disrupting E2F binding to the Phosphoglycerate kinase promoter impairs metabolism in Drosophila, indicating critical metabolic control by cell cycle...

E2F regulation of the Phosphoglycerate kinase gene is functionally important in Drosophila development

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Implication

Key Points

Cite This Study

E2F regulation of the Phosphoglycerate kinase gene is functionally important in Drosophila development

Discussion

Member takes

Implication

Key Points

Cite This Study

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