Why the study?
Understanding the spectrum of sequence variants in persistently infected animals is essential for transmission studies, but quasispecies diversity needed analysis without amplification bias.
Population
Viral RNA from the serum of a persistently infected cow and from cell culture fluid after three passages
Comparison
Serum-derived virus vs cell culture-passaged virus
Design
Comparative sequencing study
Authors
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In vitro BVDV studies may underestimate quasispecies plasticity; leaves open contributions to interspecies transmission and eradication challenges.
Cultured viruses lose quasispecies diversity rapidly and may not provide reliable molecular markers for source tracing or transmission studies.
Russell et al. (2020) studied this question.
Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context: