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March 4, 2020PathogensOpen Access

Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag

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Why the study?

Conventional serum neutralization tests for pestivirus infections require labor-intensive immunoperoxidase assays or time-consuming observation of cytopathic effects over 3 to 7 days.

Population

Classical swine fever virus, bovine viral diarrhea virus, and border disease virus

Comparison

Luciferase-based serum neutralization test vs conventional serum neutralization tests

Design

Preclinical assay development and evaluation study

Authors

MTMadoka TetsuoHokkaido UniversityKMKeita MatsunoSapporo International UniversityTTTomokazu TamuraHokkaido University

Discussion

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Implication

Luciferase-based SNT enables faster high-throughput pestivirus serology; leaves open routine replacement of conventional methods pending broader validation.

Structured PICO

P
Population
Pestiviruses (classical swine fever virus, bovine viral diarrhea virus, and border disease virus)
I
Intervention
Luciferase-based serum neutralization test (SNT) using recombinant pestiviruses possessing an 11-amino-acid subunit derived from NanoLuc luciferase
C
Comparator
Conventional SNTs (immunoperoxidase assay or observation of cytopathic effect after 3 to 7 days incubation)
O
Outcome
SNT titer determination time and comparability to conventional methodssurrogate

A novel luciferase-based serum neutralization test enables more rapid and high-throughput serological diagnosis of pestivirus infections compared to conventional methods.

Cite This Study

Tetsuo et al. (2020) studied this question.

synapsesocial.com/papers/6a7c00441f8f1eb7b8ed4e18https://doi.org/10.3390/pathogens9030188
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Also Consider

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  1. 1A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene1998 · 22 citations
  2. 2Classical Swine Fever—An Updated Review2017 · 277 citations
  3. 3The control of classical swine fever in wild boar2015 · 119 citations
  4. 4Molecular, biological, and antigenic characterization of a <i>Border disease virus</i> isolated from a pig during classical swine fever surveillance in Japan2014 · 19 citations
  5. 5Characterization and purification of recombinant bovine viral diarrhea virus particles with epitope-tagged envelope proteins2011 · 25 citations