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June 1, 1998Journal of VirologyOpen Access

A Recombinant Classical Swine Fever Virus Stably Expresses a Marker Gene

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Population

SK-6 porcine kidney cells and classical swine fever virus (CSFV) strain Alfort/187

Comparison

Insertion of bacterial chloramphenicol… vs Parental virus vA187-1

Design

Preclinical

Follow-up

10 virus passages

Authors

CMChristian MoserJTJon-Duri TratschinMHMartin Hofmann‐Apitius

Discussion

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Overview

Supports CSFV marker-virus tools for replication studies; leaves open translation to pathogenesis or vaccine models.

Structured PICO

P
Population
SK-6 porcine kidney cells and classical swine fever virus (CSFV) strain Alfort/187
I
Intervention
Insertion of bacterial chloramphenicol acetyltransferase (CAT) gene into the viral Npro gene to create recombinant virus vA187-CAT
C
Comparator
Parental virus vA187-1
O
Outcome
Viral replication, gene expression, and stability of the inserted genesurrogate

The creation of a stable recombinant classical swine fever virus expressing a CAT marker gene provides a useful tool for quantitative analysis of viral replication and gene expression.

Cite This Study

Moser et al. (1998) studied this question.

synapsesocial.com/papers/6a82f4e95c109110e17d561ehttps://doi.org/10.1128/jvi.72.6.5318-5322.1998
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA1996 · 207 citations
  2. 2Expression of a foreign protein by influenza A virus1994 · 90 citations
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  4. 4Generation of a DNA-launched classical swine fever virus infectious clone packaged in bacterial artificial chromosome2022 · 2 citations
  5. 5Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus1996 · 173 citations