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October 6, 2022Virus ResearchOpen Access

Generation of a DNA-launched classical swine fever virus infectious clone packaged in bacterial artificial chromosome

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Population

PK15 cells and bacteria

Design

Preclinical

Authors

DFDingLin FanCHCongxia HuXYXidan Yang

Discussion

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Overview

May aid CSFV research tools; leaves open vaccine applications pending in vivo validation.

Structured PICO

P
Population
PK15 cells and bacteria
I
Intervention
Construction of a full-length genome of classical swine fever virus (CSFV) Shimen strain in a bacterial artificial chromosome (BAC) vector with CMV promoter and HDV ribozyme/BGH termination signals (pBAC-smCSFV), and development of a reporter virus CSFV-mCherry
O
Outcome
Recovery of infectious progeny virus, stability of the infectious cDNA clone, and stable expression of mCherry reportersurrogate

The development of a concise and efficient CSFV infectious cDNA clone and reporter virus using a CMV promoter and BAC system provides a new tool for studying CSFV biology and vaccine development.

Cite This Study

Fan et al. (2022) studied this question.

synapsesocial.com/papers/6a82a5eaac779081c023b35ehttps://doi.org/10.1016/j.virusres.2022.198961
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Also Consider

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  1. 1Rapid Recovery of Classical Swine Fever Virus Directly from Cloned cDNA2013 · 6 citations
  2. 2Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles1996 · 153 citations
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  4. 4Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus1996 · 173 citations
  5. 5Classical swine fever virus: the past, present and future2020 · 198 citations