Key result
Targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA allowed for the efficient generation and stable maintenance of recombinant classical swine fever viruses.
Population
Porcine kidney and sheep fetal thymoid cells, E. coli DH10B cells, and classical swine fever viruses
Design
Preclinical
Authors
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May aid CSFV vaccine research in swine models; leaves open translation to veterinary practice.
Targeted recombination-mediated mutagenesis of BACs provides an efficient and stable method for the genetic manipulation and rescue of RNA viruses like CSFV.
Rasmussen et al. (2013) studied Classical swine fever virus (CSFV) / RNA virus genetics. Targeted recombination-mediated mutagenesis of BACs vs. Parental virus was evaluated on Generation and genetic stability of recombinant RNA viruses. Targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA allowed for the efficient generation and stable maintenance of recombinant classical swine fever viruses.
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