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January 21, 2025Discover Viruses.Open Access

Construction of full length cDNA clone of classical swine fever virus using an improved strategy

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Population

Cell culture-adapted lapinized Indian Classical Swine Fever virus (CSFV) genome

Design

Preclinical

Authors

PKParveen KumarPDPronab DharVUVikramadiya Upmanyu

Discussion

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Overview

Advances CSFV reverse genetics tools; leaves open applications for improved swine vaccines.

Structured PICO

P
Population
Cell culture-adapted lapinized Indian Classical Swine Fever virus (CSFV) genome
I
Intervention
Cloning into pBR322 vector using an improved method (RT-LA PCR into two overlapping fragments of ~ 6.5 kb and 5.8 kb sizes, fusion, and cloning)
O
Outcome
Successful generation of full-length cDNA clone (pBR/T7-CSFV) confirmed by PCR, restriction enzyme analysis, and nucleotide sequencingsurrogate

The construction of a full-length cDNA clone of Indian CSFV provides a reverse genetics model for creating recombinant viruses and developing DIVA strategies.

Cite This Study

Kumar et al. (2025) studied this question.

synapsesocial.com/papers/6a9276cf915bce628c4f313ehttps://doi.org/10.1007/s44370-025-00008-x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA1996 · 207 citations
  2. 2Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping2018 · 5 citations
  3. 3Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain2012 · 19 citations
  4. 4Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus1996 · 173 citations
  5. 5Classical Swine Fever Virus Biology, Clinicopathology, Diagnosis, Vaccines and a Meta-Analysis of Prevalence: A Review from the Indian Perspective2020 · 34 citations