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August 9, 2018BMC GenomicsOpen Access

Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

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Population

Classical swine fever virus from a pig infected with vKos

Design

Preclinical

Authors

CJCamille Melissa JohnstonUFUlrik FahnøeGBGraham J. Belsham

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Overview

Optimizes viral population analysis in animal models; leaves open clinical translation pending human validation.

Structured PICO

P
Population
Classical swine fever virus (CSFV) from a pig infected with vKos (rescued from a cDNA clone corresponding to the highly virulent CSFV Koslov strain)
I
Intervention
Direct molecular cloning of full-length cDNAs derived from viral RNA using long RT-PCR
O
Outcome
Generation of full-length cDNA clones and characterization of viral haplotypes

This optimized methodology enables highly efficient construction of full-length cDNA clones corresponding to individual viral genomes present within RNA virus populations.

Cite This Study

Johnston et al. (2018) studied this question.

synapsesocial.com/papers/6a82a8665b7acbbacb8d636fhttps://doi.org/10.1186/s12864-018-4971-8
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Also Consider

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  1. 1Efficient generation of recombinant RNA viruses using targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA2013 · 10 citations
  2. 2The European Classical Swine Fever Virus Database: Blueprint for a Pathogen-Specific Sequence Database with Integrated Sequence Analysis Tools2016 · 17 citations
  3. 3Heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses1992 · 54 citations