May 1, 2013Journal of Integrative AgricultureOpen Access

Rapid Recovery of Classical Swine Fever Virus Directly from Cloned cDNA

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Population

PK/T7 cells and classical swine fever virus Shimen strain

Comparison

Direct transfection of pOKShimen-RzTΦ into PK/T7… vs In vitro transcription-based rescue system

Design

Preclinical

Authors

JHJunhua HuangZhejiang A & F University YLYongfeng LiGuangxi Medical University FHFan HeCentral Connecticut State University

Discussion

Member takes

Overview

Enhances CSFV reverse genetics efficiency; leaves open whether it advances vaccine or pathogenesis studies in swine.

Structured PICO

Population

PK/T7 cells (a PK-15-derived cell line stably expressing bacteriophage T7 RNA polymerase) and classical swine fever virus (CSFV) Shimen strain

Intervention

Direct transfection of pOKShimen-RzTΦ (full-length genomic cDNA flanked by T7 promoter, HDV ribozyme, and T7 terminator) into PK/T7 cells

Comparator

In vitro transcription-based rescue system (classical reverse genetics)

Outcome

Viral rescue efficiency (time to rescue and viral titer)surrogate

Direct transfection of cloned cDNA into PK/T7 cells provides a more rapid and efficient method for recovering classical swine fever virus compared to traditional in vitro transcription methods.

Cite This Study

Huang et al. (2013) studied this question.

synapsesocial.com/papers/6a9276cf915bce628c4f3142 https://doi.org/10.1016/s2095-3119(13)60258-0

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May 1, 2013Journal of Integrative AgricultureOpen Access

Rapid Recovery of Classical Swine Fever Virus Directly from Cloned cDNA

View Full Paper

Ask AI

Bookmark

Population

PK/T7 cells and classical swine fever virus Shimen strain

Comparison

Direct transfection of pOKShimen-RzTΦ into PK/T7… vs In vitro transcription-based rescue system

Design

Preclinical

Authors

JHJunhua HuangZhejiang A & F University YLYongfeng LiGuangxi Medical University FHFan HeCentral Connecticut State University

Discussion

Member takes

Overview

Enhances CSFV reverse genetics efficiency; leaves open whether it advances vaccine or pathogenesis studies in swine.

Structured PICO

Population

PK/T7 cells (a PK-15-derived cell line stably expressing bacteriophage T7 RNA polymerase) and classical swine fever virus (CSFV) Shimen strain

Intervention

Direct transfection of pOKShimen-RzTΦ (full-length genomic cDNA flanked by T7 promoter, HDV ribozyme, and T7 terminator) into PK/T7 cells

Comparator

In vitro transcription-based rescue system (classical reverse genetics)

Outcome

Viral rescue efficiency (time to rescue and viral titer)surrogate

Direct transfection of cloned cDNA into PK/T7 cells provides a more rapid and efficient method for recovering classical swine fever virus compared to traditional in vitro transcription methods.

Cite This Study

Huang et al. (2013) studied this question.

synapsesocial.com/papers/6a9276cf915bce628c4f3142 https://doi.org/10.1016/s2095-3119(13)60258-0

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