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March 1, 1996Journal of VirologyOpen Access

Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles

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Population

Porcine cells and classical swine fever virus (CSFV)

Comparison

Construction of full-length CSFV cDNA clones and… vs Parental CSFV

Design

Preclinical

Authors

GMGregor MeyersHTHeinz‐Jürgen ThielTRTill Rümenapf

Discussion

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Overview

Establishes reverse genetics for CSFV; leaves open in vivo validation for attenuation and cytopathogenicity studies.

Structured PICO

P
Population
Porcine cells and classical swine fever virus (CSFV)
I
Intervention
Construction of full-length CSFV cDNA clones and transfection of in vitro-transcribed RNA
C
Comparator
Parental CSFV
O
Outcome
Recovery of infectious CSFV and cytopathogenic defective interfering particles (DIs)surrogate

The establishment of a reverse genetics system for CSFV enables novel approaches to analyze pestiviral molecular biology, attenuation, and cytopathogenicity.

Cite This Study

Meyers et al. (1996) studied this question.

synapsesocial.com/papers/6a7c00461f8f1eb7b8ed4e33https://doi.org/10.1128/jvi.70.3.1588-1595.1996
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Also Consider

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  1. 1Complete nucleotide sequence of infectious Coxsackievirus B3 cDNA: two initial 5' uridine residues are regained during plus-strand RNA synthesis1990 · 253 citations
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  4. 4Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus.1991 · 230 citations
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