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June 1, 1996Journal of VirologyOpen Access

Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA

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Population

Classical swine fever virus (CSFV) strain Alfort/187 genome and porcine kidney cells

Design

Preclinical

Authors

NRNicolas RuggliJTJon-Duri TratschinCMChristian Mittelholzer

Discussion

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Overview

May enable CSFV reverse genetics in swine models; leaves open clinical translation or human applications.

Structured PICO

P
Population
Classical swine fever virus (CSFV) strain Alfort/187 genome and porcine kidney cells
I
Intervention
Cloning of full-length cDNA and in vitro synthesis of full-length viral RNA by runoff transcription
O
Outcome
Infectivity of the synthesized RNA after transfection into porcine kidney cellssurrogate

The development of a stably cloned full-length CSFV cDNA allows for site-specific mutagenesis and detailed molecular characterization of the virus.

Cite This Study

Ruggli et al. (1996) studied this question.

synapsesocial.com/papers/6a7c00461f8f1eb7b8ed4e32https://doi.org/10.1128/jvi.70.6.3478-3487.1996
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