October 30, 2001Proceedings of the National Academy of SciencesOpen Access

Internal initiation in Saccharomyces cerevisiae mediated by an initiator tRNA/eIF2-independent internal ribosome entry site element

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Population

Saccharomyces cerevisiae models, including wild-type, cells with constitutive expression of eIF2 kinase…

Design

Preclinical

Authors

STSunnie R. ThompsonUniversity of Alabama at Birmingham KGKeith D. GulyasWayne State University PSPeter SarnowStanford University

Discussion

Member takes

Implication

Enables yeast genetic dissection of IRES mechanisms; leaves open translation to mammalian systems.

Structured PICO

Population

Saccharomyces cerevisiae (yeast) models, including wild-type, cells with constitutive expression of eIF2 kinase GCN2, cells with disrupted initiator tRNA(met) genes, and translation-competent extracts.

Intervention

Cricket paralysis virus internal ribosome entry site (IRES) element

Outcome

Translation of a second URA3 cistron in dicistronic mRNAs (uracil-independent growth) and tRNA(met)-independent initiation

Demonstrates that a viral IRES can function in yeast via factor-independent ribosome recruitment, enabling the use of yeast genetics to study internal ribosome entry mechanisms.

Cite This Study

Thompson et al. (2001) studied this question.

synapsesocial.com/papers/6a7c1139a540d02696c2bf97 https://doi.org/10.1073/pnas.241286698

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