What question did this study set out to answer?

To investigate the dynamics of platelet thrombus initiation by extravascular adenosine diphosphate (ADP) and evaluate how histamine affects thrombus formation in normal venules.

May 1, 1972The Journal of PhysiologyOpen Access

The initiation of platelet thrombi in normal venules and its acceleration by histamine

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Authors

NBNicola A. BegentFilm Independent GBG. V. R. BornUniversity of London DSDavid E. SharpFundación Juan March

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Implication

In vivo animal study reveals that histamine accelerates ADP-induced platelet thrombus formation in venules, suggesting endothelial cell separation promotes prothrombotic molecule diffusion.

Key Points

To investigate the dynamics of platelet thrombus initiation by extravascular adenosine diphosphate (ADP) and evaluate how histamine affects thrombus formation in normal venules.
Delivered ADP (10–400 nA, 10 mM) via micro-iontophoresis through micropipettes placed within 5 µm of cheek pouch venules in anesthetized hamsters to measure time to first platelet adherence.
Co-administered histamine (10 mM, 300 nA) or bradykinin with ADP to evaluate changes in thrombus onset latency, endothelial gap formation, and venular blood flow velocity.
ADP induced platelet thrombi with latency inversely related to current between 10 nA and 200 nA, reaching a minimum formation time of less than 20 seconds at currents of 200 nA or greater.
Histamine decreased the time to initial thrombus appearance by up to 40% compared to ADP alone without altering blood flow velocity, with latency returning to baseline within 5 minutes after stopping histamine.
Bradykinin did not cause endothelial gap formation or accelerate thrombus formation, indicating that histamine accelerates thrombosis specifically by opening endothelial junctions to facilitate ADP diffusion.

Cite This Study

Begent et al. (1972) studied this question.

synapsesocial.com/papers/6a7c1d2c48312d37e47605dc https://doi.org/10.1113/jphysiol.1972.sp009843

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Implication

In vivo animal study reveals that histamine accelerates ADP-induced platelet thrombus formation in venules, suggesting endothelial cell separation promotes prothrombotic molecule diffusion.

The initiation of platelet thrombi in normal venules and its acceleration by histamine

Discussion

Member takes

Implication

Key Points

Cite This Study

The initiation of platelet thrombi in normal venules and its acceleration by histamine

Discussion

Member takes

Implication

Key Points

Cite This Study

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