Bacteria Isolation from Three Species of Marine Sponge

In this study, three sponge species (red, blue, and white) were collected from sea squirts, mussels, and oysters in Qixing Bay, Shenzhen. Bacteria were extracted from sponge tissues using seawater, plated on Marine Agar 2216E, and purified. The 16S rDNA of purified bacterial isolates was amplified by PCR and subjected to Sanger Sequencing. Based on the 16S rDNA sequences, we identified three bacteria: Tenacibaculum mesophilum, Pseudoalteromonas sp., and Pseudovibrio sp. from the three types of marine sponges.