What question did this study set out to answer?

Formulate a biophysical model to evaluate how intracellular calcium entry and submembrane accumulation regulate calcium channel inactivation.

December 22, 1982Proceedings of the Royal Society B Biological Sciences

A binding-site model for calcium channel inactivation that depends on calcium entry

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Authors

NSN. B. StandenLa Trobe University PSPeter StanfieldGoogle (United States)

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Implication

Model supports submembrane calcium-dependent inactivation in cardiac channels; leaves open necessity of voltage-dependent mechanisms pending experimental validation.

Key Points

Formulate a biophysical model to evaluate how intracellular calcium entry and submembrane accumulation regulate calcium channel inactivation.
Constructed a mathematical binding-site model where calcium entering through the cell membrane accumulates in a submembrane compartment and binds an internal site to induce inactivation.
Incorporated simplifying kinetic assumptions for intracellular calcium buffering and clearance mechanisms.
Simulated single-pulse and double-pulse voltage-clamp experimental protocols to test model predictions against observed channel kinetics.
Simulations replicated single- and double-pulse voltage-clamp dynamics, accurately yielding non-exponential calcium current turn-off.
Interventions that slowed inactivation rates produced a predicted increase in peak inward calcium current.
Two-phase current decay and incomplete current recovery during two-pulse experiments were reproduced without invoking voltage-dependent inactivation or multiple channel subtypes.

Cite This Study

Standen et al. (1982) studied this question.

synapsesocial.com/papers/6a7c21e23469ba7c45ed2a4e https://doi.org/10.1098/rspb.1982.0097

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Implication

Model supports submembrane calcium-dependent inactivation in cardiac channels; leaves open necessity of voltage-dependent mechanisms pending experimental validation.

A binding-site model for calcium channel inactivation that depends on calcium entry

Discussion

Member takes

Implication

Key Points

Cite This Study

A binding-site model for calcium channel inactivation that depends on calcium entry

Discussion

Member takes

Implication

Key Points

Cite This Study

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