Key result
Microfluidic nested PCR followed by MiSeq sequencing enabled simultaneous high-throughput detection and genotyping of 11 human pathogenic RNA viruses with a detection limit of 100 to 10^3 copies/µL in cDNA.
Population
Standard DNA samples, artificially contaminated oysters, human fecal samples, and sewage samples
Design
Other
Authors
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May support multiplex RNA virus surveillance in clinical samples; leaves open prospective validation before adoption.
Microfluidic nested PCR followed by MiSeq sequencing enables efficient, high-throughput detection and genotyping of multiple human pathogenic RNA viruses in environmental and clinical samples.
Oshiki et al. (2018) studied Human pathogenic RNA viruses. Microfluidic nested PCR and MiSeq amplicon sequencing (MFnPCR-MiSeq) was evaluated on Detection limit and genotyping capability for 11 human RNA viruses. Microfluidic nested PCR followed by MiSeq sequencing enabled simultaneous high-throughput detection and genotyping of 11 human pathogenic RNA viruses with a detection limit of 100 to 10^3 copies/µL in cDNA.
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