Key result
A modified RNA-Seq approach successfully recovered >97% of reference genomes for Norovirus and Hepatitis C virus directly from clinical samples, with high concordance to Sanger sequencing.
Population
80 Norovirus samples from faeces and 2 Hepatitis C virus samples from blood.
Comparison
RNA shotgun sequencing using Illumina MiSeq and… vs Sanger sequencing (amplicon-based).
Design
Other
Authors
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May enable culture-free RNA virus WGS in clinical labs; leaves open diagnostic utility pending prospective validation.
A novel RNA-Seq approach enables accurate, high-throughput whole genome sequencing of RNA viruses directly from clinical samples without the need for culture or target-specific amplification.
Batty et al. (2013) studied Norovirus and Hepatitis C virus infections (n=82). Modified RNA-Seq approach vs. Sanger sequencing was evaluated on Recovery of reference genomes and nucleotide differences compared to Sanger sequencing. A modified RNA-Seq approach successfully recovered >97% of reference genomes for Norovirus and Hepatitis C virus directly from clinical samples, with high concordance to Sanger sequencing.
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