What question did this study set out to answer?

To establish and validate rapid, high-throughput neutralization assays to quantify SARS-CoV-2 neutralizing antibody titers for clinical assessment and vaccine development.

November 20, 2020Current Protocols in ImmunologyOpen Access

Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS‐CoV‐2 Neutralizing Antibodies

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To establish and validate rapid, high-throughput neutralization assays to quantify SARS-CoV-2 neutralizing antibody titers for clinical assessment and vaccine development.
Designed a basic focus reduction neutralization test (FRNT) utilizing immunostaining of infected cell monolayers with a chromogen deposit readout.
Developed an alternate FRNT protocol utilizing an infectious clone-derived SARS-CoV-2 reporter virus expressing mNeonGreen (FRNT-mNG).
Both the chromogenic FRNT and fluorescent FRNT-mNG platforms successfully measure antibody neutralization capacity against SARS-CoV-2.
The optimized protocols provide high-throughput capacity compatible with large-scale serological surveillance and clinical vaccine trials.

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Implication

Protocol study demonstrates rapid focus reduction neutralization assays for SARS-CoV-2 antibodies, highlighting utility for high-throughput vaccine evaluation.

Cite This Study

Vanderheiden et al. (2020) studied this question.

synapsesocial.com/papers/6a7c4d4ebca1f9b7b0a163f0 https://doi.org/10.1002/cpim.116

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Implication

Protocol study demonstrates rapid focus reduction neutralization assays for SARS-CoV-2 antibodies, highlighting utility for high-throughput vaccine evaluation.

Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS‐CoV‐2 Neutralizing Antibodies

Key Points

Discussion

Implication

Cite This Study

Development of a Rapid Focus Reduction Neutralization Test Assay for Measuring SARS‐CoV‐2 Neutralizing Antibodies

Key Points

Discussion

Implication

Cite This Study

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