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manner of reading and recording is described under Results and in the footnote to Table I.Staphylococci were tested by the same method (including readings at 42 hours on 579 strains) for sensitivity to penicillin, tetracycline, chloramphenicol, and novobiocin.A number of strains, representing different patterns of resistance in the ditch plate test, were tested by tube dilution and plate dilution methods; doubling dilutions of the antibiotics were incorporated in nutrient agar or broth, and these were inoculated with undiluted and with a 1/500 dilution of overnight broth culture, one drop (0.02 ml.) in the tube test and a loopful in the plate test.
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Lowbury et al. (1959) studied this question.
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