Proteochondroitin sulfate synthesis in subcultured chick embryo tibial chondrocytes.

The PCS-H and PCS-L nomenclature used here is that used by Kimata et al. (1) for heavy and light PCS fractions that are separated by sucrose density gradient sedimentation.PCS-H and PCS-L correspond to peaks I and IV, respectively, in the sucrose gradients of Okayama et al. (4) and to the PCS fractions, which emerge early and late, respectively, when these components are separated by gel fltration on 1% agarose (3) or Sepharose 2B (5).