August 18, 2004Journal of Cell Science
Basolateral localisation of KCNQ1 potassium channels in MDCK cells: molecular identification of an N-terminal targeting motif
Population
Polarised MDCK cells expressing KCNQ1 potassium channels
Comparison
Mutation of tyrosine 51 or di-leucine-like motif… vs Wild-type KCNQ1 channels
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Preclinical only; leaves open whether these motifs influence KCNQ1 function in human cardiac or renal disease.
Structured PICO
P
Population
Polarised MDCK cells expressing KCNQ1 potassium channels
I
Intervention
Mutation of tyrosine 51 or di-leucine-like motif (LEL) at residues 38-40 in the N-terminal tail of KCNQ1; co-expression of KCNE beta-subunits
C
Comparator
Wild-type KCNQ1 channels
O
Outcome
Subcellular localisation of KCNQ1 channelssurrogate
This study identifies specific N-terminal motifs (tyrosine 51 and a di-leucine-like motif) responsible for the basolateral localization of KCNQ1 potassium channels in epithelial cells.
Cite This Study
Jespersen et al. (2004) studied this question.