December 28, 2002Cardiovascular ResearchOpen Access

Increased Ca-sensitivity of the contractile apparatus in end-stage human heart failure results from altered phosphorylation of contractile proteins

View Full Paper

Ask AI

Bookmark

Population

Single left ventricular myocytes from non-failing donor (n=6) and end-stage failing (n=10) human hearts

Comparison

Analysis of isoform composition and… vs Myocytes from non-failing donor hearts

Design

Preclinical

Authors

ZPZoltán PappSemmelweis University RZR. ZarembaUniversity Medical Center NBNicky M. BoontjeAmsterdam UMC Location Vrije Universiteit Amsterdam

Discussion

Member takes

Implication

Hypothesis-generating in animal HF models; leaves open translation to human disease mechanisms or therapies.

Structured PICO

Population

Single left ventricular myocytes from non-failing donor (n=6) and end-stage failing (n=10) human hearts

Intervention

Analysis of isoform composition and phosphorylation status of contractile proteins, and incubation with protein kinase A

Comparator

Myocytes from non-failing donor hearts

Outcome

Isometric force and its Ca(2+)-sensitivity (pCa50)surrogate

Increased Ca2+-responsiveness in end-stage failing human hearts is driven by changes in the phosphorylation status of MLC-2 and TnI rather than a shift in contractile protein isoforms.

Cite This Study

Papp et al. (2002) studied this question.

synapsesocial.com/papers/6a7c837bf60042df986e9c5d https://doi.org/10.1016/s0008-6363(02)00606-5

Topics

Heart failure HFrEF treatment

View Full Paper

Ask AI

Bookmark

Increased Ca-sensitivity of the contractile apparatus in end-stage human heart failure results from altered phosphorylation of contractile proteins

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Topics

Increased Ca-sensitivity of the contractile apparatus in end-stage human heart failure results from altered phosphorylation of contractile proteins

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider