Glucose‐Limited fed‐batch cultivation of Escherichia coli with computer‐controlled fixed growth rate

Growth cessation of Escherichia coli in nondialysis fedbatch culture at biomass densities over 30-40 g/L has been shown to be the result of the accumulation of acetate, lactate, pyruvate, succinate, propionate, and isobutyrate in the culture broth.' This conclusion was supported by the fact that high cell concentrations (140 g/L) and cellular yields were achieved in dialysis fed-batch culture of E. coli.