Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy

Figures

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  Figure 1. Left ventricular wall of HCM cat visualized by two dimensional echocardiography in the (A) short axis view, (B) color flow doppler in long axis view, and (C) the motion mode (M mode) in long axis view. IVS, interventricular septum; LVPW, left ventricular proximal wall; LV, left ventricle; LA, left atrium; AO, aorta.

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  Figure 2. The results of MYBPC3-A31P and A74T polymorphism from genotyping by sequencing. (A) Gel electrophoresis was represented 242 bp of target DNA of MYBPC3 gene in lane 2 and 3. (B) The sanger sequencing result of MYBPC3-A31P was showed in three group including homozygous wild-type (G/G), heterozygous mutation (G/C), and homozygous mutation (C/C). The green arrow pointed 2 peaks of guanine and cytosine and the red arrow pointed of only cytosine. (C) MYBPC3-A74T sequencing result was showed in three group including homozygous wild-type (G/G), heterozygous mutation (G/A), and homozygous mutation (A/A). The blue arrow pointed 2 peaks of guanine and alanine while the yellow arrow represented one peak of alanine.

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  Figure 3. Principal component analysis shows Maine Coon cats samples segregate from control cats. A three-dimensional scatterplot plot of non-gene mutation, gene mutation (A31P and A74T), and left ventricular wall hypertrophy cats (LV wall Thickening).

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  Figure 4. (A) The diagram represents overlap among protein types with 5,946 identified proteins. (B) Protein and drug interaction networks of proteins dominate in sarcomeric gene mutation cats.

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  Figure 5. Protein identification by MALDI-TOF analysis. Mass spectrometry of the protein isolated from the 2D gels of protein extracts of cat serum in domestic shorthair cats (Control), Maine Coon cat, and Maine Coon with sarcomeric gene mutation cats.