Key result
Low-level laser irradiation significantly altered 4 cardiac cytokines (including GM-CSF and fractalkine) at 1 day post-MI and reduced infarct area expansion at 2 weeks without improving heart function.
RCT (n=94)
Randomized
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OBJECTIVES: Low-level laser irradiation (LLLI) has the potential of exerting cardioprotective effect following myocardial infarction (MI). The authors hypothesized that LLLI could influence the expression of cardiac cytokines and contribute to the reversal of ventricular remodeling. BACKGROUND: LLLI regulates the expression of cytokines after tissue damage. However, little is known concerning the alteration of the cardiac cytokine expression profile after LLLI. METHODS: MI was created by coronary ligation. The surviving rats were divided randomly into laser and control groups. 33 rats were exposed to a diode laser (635 nm, 5 mW, CW, laser, beam spot size 0.8 cm(2), 6 mW/cm(2), 150 sec, 0.8 J, 1J/cm(2)) as laser group. Another 33 rats received only coronary ligation and served as control group. 28 rats received a thoracotomy without coronary ligation (sham group). One day after laser irradiation, 5 rats from each group were sacrificed and the heart tissues were analyzed by cytokine antibody arrays. Enzyme-linked immunosorbent assay (ELISA) was performed to confirm its reliability. Two weeks after MI, cardiac function and structure were evaluated by echocardiography and histological study. RESULTS: Cytokine antibody array indicated 4 cytokines were significantly changed after laser therapy. ELISA confirmed that granulocyte-macrophage colony stimulating factor and fractalkine were the cytokines involved in the response to therapeutic laser irradiation. However, there was no difference in cytokine release between various groups at 2 weeks after MI. Although LLLI did not improve the damaged heart function, it did reduce the infarct area expansion. CONCLUSIONS: The antibody-based protein array technology was applied for screening the cytokine expression profile following MI, with or without laser irradiation. The expression of multiple cytokines was regulated in the acute phase after LLLI. Our results revealed a potential novel mechanism for applying laser therapy to the treatment of heart disease.
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Yang et al. (2011) conducted an RCT in Acute myocardial infarction (rat model) (n=94). Low-level laser irradiation (LLLI) vs. Coronary ligation control without laser (plus sham thoracotomy group) was evaluated on Cardiac cytokine expression profile and ventricular remodeling (cardiac function and infarct area expansion). Low-level laser irradiation significantly altered 4 cardiac cytokines (including GM-CSF and fractalkine) at 1 day post-MI and reduced infarct area expansion at 2 weeks without improving heart function.
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