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设计并研制了一套体积为30 cm×30 cm×40 cm的紧凑型多色光片荧光显微镜,使用均值漂移超分辨算法将成像分辨率从530 nm降低至330 nm,通过对荧光小球、Argolight商用荧光显微镜分辨率标定板、菠菜根切片和鼠肠切片进行成像实验验证了系统的成像性能。本系统结构紧凑,可以放置于生物医学实验平台上使用,并且能够对活体样品进行长时间超分辨三维多色荧光成像,为神经科学、发育生物学等生物医学领域提供一种简洁易用的显微成像工具。
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Hao et al. (2025) studied this question.
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