October 1, 2000RNAOpen Access

De novo synthesis of minus strand RNA by the rotavirus RNA polymerase in a cell-free system involves a novel mechanism of initiation

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Population

Cell-free replication system using rotavirus open cores and viral (+) strand RNA

Design

Preclinical

Authors

Discussion

Advances understanding of rotavirus replication; leaves open antiviral translation from animal models.

Population

Cell-free replication system using rotavirus open cores and viral (+) strand RNA

Intervention

Preincubation with GTP, ATP, CTP, or UTP and addition of NaCl

Outcome

Synthesis of (-) strand RNA (dsRNA products)

The study elucidates a novel mechanism of initiation for rotavirus (-) strand RNA synthesis involving a 5'-phosphorylated dinucleotide.

Chen et al. (2000) studied this question.

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