Why the study?
Live cardiomyocyte isolation techniques prior to fixation show inherent sample-to-sample fluctuations in yield, viability, and quality despite optimization.
Population
Adult mammalian hearts
Comparison
Cardiomyocyte isolation involving fixation prior to enzymatic digestion
Design
Preclinical protocol development and validation study
Authors
Loading...
May enhance cardiomyocyte isolation for immunostaining; leaves open standardization and validation across models.
A novel protocol for isolating cardiomyocytes from fixed hearts improves yield and preserves morphology for downstream immunocytochemistry and ploidy analysis.
Yücel et al. (2020) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: