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Investigate the molecular identity and developmental functions of the TRANSPORT INHIBITOR RESPONSE2 (TIR2) gene in plant auxin biosynthesis.

July 22, 2009PLANT PHYSIOLOGYOpen Access

The TRANSPORT INHIBITOR RESPONSE2 Gene Is Required for Auxin Synthesis and Diverse Aspects of Plant Development

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Investigate the molecular identity and developmental functions of the TRANSPORT INHIBITOR RESPONSE2 (TIR2) gene in plant auxin biosynthesis.
Assessed growth phenotypes of tir2 plant mutants following treatment with 5-methyl-tryptophan, indole-3-pyruvic acid, and indole-3-acetic acid.
Conducted molecular cloning and sequence characterization to identify the TIR2 locus.
Evaluated TIR2 expression patterns under temperature changes and during root gravitropic stimulation.
Molecular characterization identified TIR2 as identical to TAA1, which encodes a tryptophan aminotransferase functioning in the indole-3-pyruvic acid auxin biosynthetic pathway.
Application of indole-3-pyruvic acid and indole-3-acetic acid rescued the tir2 short hypocotyl phenotype, whereas mutants demonstrated hypersensitivity to the tryptophan inhibitor 5-methyl-tryptophan.
TIR2 expression was induced on the lower side of gravitropically responding roots and required for temperature-dependent hypocotyl elongation, defining its role in environmental growth regulation.

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Implication

Genetic analysis demonstrates that TIR2 encodes a tryptophan aminotransferase essential for auxin synthesis in plants, indicating its central role in environmental and gravitropic growth responses.

Cite This Study

Yamada et al. (2009) studied this question.

synapsesocial.com/papers/6a7cb71b93373eeacd190d6d https://doi.org/10.1104/pp.109.138859

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Implication

Genetic analysis demonstrates that TIR2 encodes a tryptophan aminotransferase essential for auxin synthesis in plants, indicating its central role in environmental and gravitropic growth responses.

The TRANSPORT INHIBITOR RESPONSE2 Gene Is Required for Auxin Synthesis and Diverse Aspects of Plant Development

Key Points

Discussion

Implication

Cite This Study

The TRANSPORT INHIBITOR RESPONSE2 Gene Is Required for Auxin Synthesis and Diverse Aspects of Plant Development

Key Points

Discussion

Implication

Cite This Study

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