What question did this study set out to answer?

Determine the buoyant density behavior and nucleotide composition relationships of natural and synthetic DNAs in neutral and alkaline cesium salt solutions.

June 1, 1972Journal of Biological ChemistryOpen Access

Buoyant Density Studies on Natural and Synthetic Deoxyribonucleic Acids in Neutral and Alkaline Solutions

View Full Paper

Ask AI

Bookmark

Authors

RWRobert D. WellsUniversity of Bern JLJacquelynn E. LarsonTexas A&M Health Science Center

Discussion

Member takes

Implication

In vitro centrifugation analysis reveals distinct buoyant density shifts in CsCl and Cs2SO4 solutions across DNA variants, indicating density changes cannot predict nucleotide composition.

Key Points

Determine the buoyant density behavior and nucleotide composition relationships of natural and synthetic DNAs in neutral and alkaline cesium salt solutions.
Performed equilibrium buoyant density centrifugation in neutral and alkaline solutions of cesium chloride (CsCl) and cesium sulfate (Cs2SO4).
Tested 11 synthetic DNA polymers alongside 12 naturally occurring DNA samples.
No clear correlation exists between DNA nucleotide composition and the magnitude of density shifts upon alkaline titration, preventing composition prediction in single-stranded DNA.
Alkaline titration caused net buoyant density decreases for natural DNAs and certain DNA polymers in Cs2SO4 solutions, whereas CsCl solutions produced only density increases.

Cite This Study

Wells et al. (1972) studied this question.

synapsesocial.com/papers/6a7cbf301c66e457e78cebe9 https://doi.org/10.1016/s0021-9258(19)45154-5

View Full Paper

Ask AI

Bookmark

Implication

In vitro centrifugation analysis reveals distinct buoyant density shifts in CsCl and Cs2SO4 solutions across DNA variants, indicating density changes cannot predict nucleotide composition.

Buoyant Density Studies on Natural and Synthetic Deoxyribonucleic Acids in Neutral and Alkaline Solutions

Discussion

Member takes

Implication

Key Points

Cite This Study

Buoyant Density Studies on Natural and Synthetic Deoxyribonucleic Acids in Neutral and Alkaline Solutions

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider