What question did this study set out to answer?

To determine the subunit stoichiometry and receptor-binding properties of membrane-bound lymphotoxin complexes expressed on human lymphocytes.

January 1, 1995The Journal of Immunology

Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors.

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Authors

JBJeffrey L. BrowningBoston University IDIrene DougasBiogen (United States)ANApinya Ngam-ekBiogen (United States)

Discussion

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Implication

In vitro study reveals predominant LT-alpha 1/beta 2 heteromers on activated human T lymphocytes, indicating specialized signaling via the lymphotoxin-beta receptor.

Key Points

To determine the subunit stoichiometry and receptor-binding properties of membrane-bound lymphotoxin complexes expressed on human lymphocytes.
Evaluated cell-surface lymphotoxin on PMA-activated human II-23 T-cell hybridoma cells, transfected Chinese hamster ovary cells, and lymphoid tumor lines.
Characterized surface complexes using panels of subunit-specific anti-LT-alpha and anti-LT-beta monoclonal antibodies alongside soluble p55-TNF and LT-beta receptors.
Identified an early minor membrane heterotrimer of LT-alpha 2/beta 1 recognized by p55-TNF receptor and a predominant LT-alpha 1/beta 2 heterotrimer recognized by the LT-beta receptor.
Observed that neither heteromeric surface complex nor putative LT-beta homotrimers are released into the extracellular space as secreted proteins.

Cite This Study

Browning et al. (1995) studied this question.

synapsesocial.com/papers/6a7cc2f1d0b007fc43cf8193 https://doi.org/10.4049/jimmunol.154.1.33

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January 1, 1995The Journal of Immunology

Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors.

View Full Paper

Ask AI

Bookmark

Authors

JBJeffrey L. BrowningBoston University IDIrene DougasBiogen (United States)ANApinya Ngam-ekBiogen (United States)

Discussion

Member takes

Implication

In vitro study reveals predominant LT-alpha 1/beta 2 heteromers on activated human T lymphocytes, indicating specialized signaling via the lymphotoxin-beta receptor.

Key Points

To determine the subunit stoichiometry and receptor-binding properties of membrane-bound lymphotoxin complexes expressed on human lymphocytes.
Evaluated cell-surface lymphotoxin on PMA-activated human II-23 T-cell hybridoma cells, transfected Chinese hamster ovary cells, and lymphoid tumor lines.
Characterized surface complexes using panels of subunit-specific anti-LT-alpha and anti-LT-beta monoclonal antibodies alongside soluble p55-TNF and LT-beta receptors.
Identified an early minor membrane heterotrimer of LT-alpha 2/beta 1 recognized by p55-TNF receptor and a predominant LT-alpha 1/beta 2 heterotrimer recognized by the LT-beta receptor.
Observed that neither heteromeric surface complex nor putative LT-beta homotrimers are released into the extracellular space as secreted proteins.

Cite This Study

Browning et al. (1995) studied this question.

synapsesocial.com/papers/6a7cc2f1d0b007fc43cf8193 https://doi.org/10.4049/jimmunol.154.1.33

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