Antigenic Relationships of 14 Treponemes Demonstrated by Immunofluorescence

14 cultivable treponemes (Reiter, English Reiter, Kazan, Kazan numbers 2, 4, 5, and 8, Nichols, Noguchi, three oral treponemes MRB, FM, and N-39, B. vincentii, Treponema zuelzerae)

Immunofluorescence using antisera prepared versus each treponemal strain, conjugated with fluorescein isothiocyanate, and absorption with Reiter or Borrelia vincentii treponemes

Antigenic grouping based on shared group-specific antigenssurrogate